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Sifat Antirayap Resin Damar Mata Kucing dari Shorea javanica K. et V. Antitermic Properties of Resin from Shorea javanica K. et V Rita Kartika Sari; Wasrin Syafii; Kurnia Sofyan; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 2, No 1 (2004): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

This study was undertaken primarily to isolate and identify substances from resin of Shorea javanica K. et. V. that might be responsible to inhibit the termite activity of Coptotermes curvignathus Holmgren. Approximately 500 gram of dried-resin was extracted with acetone solvent. The acetone extract was then fractionated into n-hexane, diethyl ether, ethyl acetate, and insoluble fraction (residue). The no-choice bioassay test was carried-out by treating paper discs with extracts at the concentration of 2.0%, 4.0%, 6.0%, 8.0%, 10.0%, 12.0% (W/W), and 0.0 % as control. The bioassay test showed that n-hexane and diethyl ether soluble fraction exhibited highest antitermic properties at Lethal Concentration (LC)50 value of 1.62% and continuation fraction showed N3 of n-hexane fraction an EE1 of diethyl ether fraction exhibited highest toxicity at LC50 value of 1.23 % and 1.65%. Further investigation of the n-hexane soluble fraction by using CC, GC-MS, FTIR, UV-Vis, and NMR led to the isolation and identification of the main compound, namely friedelin, while the diethyl ether soluble fraction contains vulgarol B; 3,4-Secodamar-4(28)-en-3-oic acid; (7R,10S)-2,6,10-Trimethyl-7, 10–epoxy-2,11-dodecadien; and junipene (decahydro-4,8,8-trimethyl-1,4-methanoazulene)

Chemical Composition and Anticancer Activity Of the Essential Oil Of Surian Heartwood Rita K Sari; Wasrin Syafii; Suminar S Achmadi; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 9, No 2 (2011): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Chemical constituents of the essential oil of water and steam distillates of surian heartwood (Toona sinensis) were analyzed by a gas chromatograph-mass spectrometer. Then, the oil was investigated in vitro anticancer bioassay for its possible antioxidant activity by DPPH free radical scavenging and antiproliferative effects by MTT method using Raji lymphoma cancer cell lines (ATCC CCL 86), HeLa cervical cancer cell lines (ATCC CCL2), and Vero normal cell lines (ATCC CCL 81). The essential oil yield from water and steam distillates of surian heartwood was 0.4% (w/w). Thirty-two compounds were identified, that consisted mainly of oxygenated sesquiterpenes (77%). The most representative compounds were spathulenol (21%), isospathulenol (7%), α-cadinol (7%), β-cedrenoxide (6%), and torreyol (5%). The oil showed less effective antioxidant, but the oil had high antiproliferative effects on Raji and HeLa cancer cells with IC50 28 and 134 μg ml-1, respectively when compared to Vero normal cells (IC50 1412 μg ml-1). Our findings suggest that the essential oil of surian heartwood might be considered as a potentially anticancer agent on human cancer cells, especially lymphoma cancer cells.Key words: antioxidant activity, antiproliferative effects, cancer cell lines, essential oil, Toona sinensis

Sifat Anti Jamur Kayu Kupa (Syzygium polycephalum (Mig)) (Antifungal Properties of Kupa Wood (Syzygium polycephalum Mig.)) Renhart Jemi; Wasrin Syafii; Fauzi Febrianto; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 8, No 2 (2010): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The objectives of this research were to evaluate the extractive substances of heartwood of Kupa wood (Syzygium polycephalum (Mig)) and its potention as bio-active substance to wood destroying fungi i.e., Schizophyllum commune Fr and Pleurotus sp. Successive extraction method was used in this experiment. The heartwood part was then converted into 40 mesh wood flour and followed by extraction using methanol, chloroform, ethyl acetate, n-hexane and buthanol solvents. The extractives obtained were subjected to wood destroying fungi S. commune and Pleurotus sp. The results indicated that the extractive content of S. polychephalum mostly dominated by substance diluted in chloroform (2.87%), followed by ethyl acetate (0.38%), n-hexane (0.33%) and buthanol (0.05%). All the wood extracts of S. polychephalum potentially contain anti-fungal compound to inhibit the growth of S. commune Fr and Pleurotus sp fungi. N-hexane and ethyl acetate extracts of S. polychephalum are the most active extracts. Isolation of ethyl acetate fraction resulted in nine (9) active compounds (G.1-G.9) that could inhibit the growth both S. commune and Pleurotus sp with IC(50) values 49.33-61.71 ppm and 48.84-64.61 ppm, respectively. It was found that G.2 compound of ethyl acetate has anti fungal substance namely 3-O-glucosyl-3’,4’ 5-trihydroxyflavonol.

Sifat Anti Jamur Kayu Kupa (Syzygium polycephalum (Mig)) (Antifungal Properties of Kupa Wood (Syzygium polycephalum Mig.)) Renhart Jemi; Wasrin Syafii; Fauzi Febrianto; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 8, No 2 (2010): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The objectives of this research were to evaluate the extractive substances of heartwood of Kupa wood (Syzygium polycephalum (Mig)) and its potention as bio-active substance to wood destroying fungi i.e., Schizophyllum commune Fr and Pleurotus sp. Successive extraction method was used in this experiment. The heartwood part was then converted into 40 mesh wood flour and followed by extraction using methanol, chloroform, ethyl acetate, n-hexane and buthanol solvents. The extractives obtained were subjected to wood destroying fungi S. commune and Pleurotus sp. The results indicated that the extractive content of S. polychephalum mostly dominated by substance diluted in chloroform (2.87%), followed by ethyl acetate (0.38%), n-hexane (0.33%) and buthanol (0.05%). All the wood extracts of S. polychephalum potentially contain anti-fungal compound to inhibit the growth of S. commune Fr and Pleurotus sp fungi. N-hexane and ethyl acetate extracts of S. polychephalum are the most active extracts. Isolation of ethyl acetate fraction resulted in nine (9) active compounds (G.1-G.9) that could inhibit the growth both S. commune and Pleurotus sp with IC(50) values 49.33-61.71 ppm and 48.84-64.61 ppm, respectively. It was found that G.2 compound of ethyl acetate has anti fungal substance namely 3-O-glucosyl-3’,4’ 5-trihydroxyflavonol.

Antifungal Properties of Palepek Baringin Wood (Shorea leavis Ridl) Renhart Jemi; Wasrin Syafii; Fauzi Febrianto; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 10, No 1 (2012): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The objectives of this research were to evaluate the extractive substances of heartwood of palepek baringin wood (Shorea leavis Ridl) as a bio-active substance to wood destroying fungi i.e. Schizophyllum commune Fr and Pleurotus ostreatus. The 40 mesh of heartwood meals was extracted by methanol, chloroform, ethyl acetate, n-hexane and buthanol. The extractives obtained were subjected to wood destroying fungi S. commune and P. ostreatus, and preservative containing copper, chrome and boron (CCB) was used for comparison. The extractive of palepek baringin wood was dominated by ethylacetate fraction (3.54%), followed by chloroform and butanol fraction 1.73% and n-hexane (0.09%). All fractions have an antifungal activity to inhibit the growth of S. commune and P. ostreatus. Concentration of 50 ppm butanol fraction inhibited the growth of S. commune, and concentration of 250 ppm chloroform fraction inhited the growth of Pleurotus sp. Preservative of CCB 100 ppm inhibited the growth of S. commune and P. ostreatus by 43%. Butanol fraction contained 16 active compounds (PB.1-PB.16) that could inhibit the growth both of S. commune and P. ostreatus with IC50 54.29-64.32 and 48.40-65.86 ppm, respectively. Based on mass spectrometry (LC-MS) and 1HNMR analysis, the compound of PB.1.1 which has anti-fungal activity was 2,3-dihydroxyoctadecanoic acid.Key words: 2,3-dihydroxyoctadecanoic acid, antifungal, Pleurotus ostreatus, Schyzophlum commune, Shorea leavis

Anticancer Activity and Chemical Compounds of Suren Heartwood Extract (Toona sureni) Rita K Sari; Wasrin Syafii; Suminar A Achmadi; Muhammad Hanafi; Yanotama T Laksana
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 10, No 1 (2012): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The aims of this research were to determine the yield extracts from continuous extraction of suren heatwood (Toona sureni) in n-hexane, ethyl acetate and methanol solvents, to evaluate this extracts using in vitro anticancer tests (antioxidant, brine shrimp lethality test /BSLT, antiproliferative effects to HeLa servical cancer cell lines, Raji limphoma cancer cell lines, and Vero normal cell lines), and to analyze the best extract based on the anticancer activities. This experiment showed that the yield of methanolic, ethyl acetate, and n-hexane extracts were 0.43%, 0.25%, and 0.18% respectively. The methanolic and ethyl acetate extracts had the high antioxidant activities with EC50 51 and 68 μg ml-1 respectively. Based on BSLT, the ethyl acetate extract was the most active extract (LC50 40 μg ml-1), it followed by the methanolic extract (LC50 116 68 μg ml-1 ), and the n-hexane extract (LC50 161 68 μg ml-1). Further testing showed that the ethyl acetate extract had high antiproliferative effects to Raji (IC50 31 μg ml-1) and HeLa (IC50 65 μg ml-1), but it was more secure against Vero cell lines (IC50 105 μg ml-1). Whereas compounds such as catechol, linalool, and sitosterol contributed to the high anticancer activities of this ethyl acetate extract.Key words: anticancer activity, HeLa cell lines, Raji cell lines, Toona sureni, Vero cell lines

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